AS4 ("antisense 4", a transcript between Mirn184 and Rasgrf1) showed exclusive paternal expression
in brain (Nomura
T, 2008). No allelic differences
in methylation were observed in CpGs in and around this sequence.
AS4 was detected by primers that cover chr9:89,697,588-89,697,874 of NCBI mouse assembly 37 (July