A CpG island (CpG#112) 4 kb downstream of exon 1 of TSPEAR (thrombospondin-type laminin G domain and
EAR repeats) was maternally methylated in peripheral blood leukocytes and placenta. Differential
methylation was detected by PCR following DNA digestion with either HpaII or McrBC (methylation
sensitive and dependent restriction enzymes respectively) (Yamada
Y et al 2004).